GrippeCanadaV4, Main, Exploration, bibRecord, 000143

Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolated in British Columbia, Canada, 2015.

Identifieur interne : 000143 ( Main/Exploration ); précédent : 000142; suivant : 000144

Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolated in British Columbia, Canada, 2015.

Auteurs : Yohannes Berhane [Canada] ; Darwyn Kobasa [Canada] ; Carissa Embury-Hyatt [Canada] ; Brad Pickering [Canada] ; Shawn Babiuk [Canada] ; Tomy Joseph [Canada] ; Victoria Bowes [Canada] ; Mathew Suderman [Canada] ; Anders Leung [Canada] ; Colleen Cottam-Birt [Canada] ; Tamiko Hisanaga [Canada] ; John Pasick [Canada]

Source :

Scientific reports [ 2045-2322 ] ; 2016.

RBID : pubmed:26988892

Descripteurs français

KwdFr :
- Animaux (MeSH), Animaux sauvages (MeSH), Canards (virologie), Charge virale (MeSH), Colombie-Britannique (MeSH), Délétion de séquence (MeSH), Infections à Orthomyxoviridae (virologie), Oies (virologie), Phylogenèse (MeSH), Poulets (virologie), Sialidase (génétique), Souris (MeSH), Sous-type H5N1 du virus de la grippe A (génétique), Sous-type H5N1 du virus de la grippe A (isolement et purification), Suidae (MeSH), Séquence d'acides aminés (MeSH), Tropisme viral (MeSH), Virus recombinants (génétique), Virus recombinants (physiologie), Évolution moléculaire (MeSH).
MESH :
- génétique : Sialidase, Sous-type H5N1 du virus de la grippe A, Virus recombinants.
- isolement et purification : Sous-type H5N1 du virus de la grippe A.
- physiologie : Virus recombinants.
- virologie : Canards, Infections à Orthomyxoviridae, Oies, Poulets.
- Animaux, Animaux sauvages, Charge virale, Colombie-Britannique, Délétion de séquence, Phylogenèse, Souris, Suidae, Séquence d'acides aminés, Tropisme viral, Évolution moléculaire.

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Animals, Wild (MeSH), British Columbia (MeSH), Chickens (virology), Ducks (virology), Evolution, Molecular (MeSH), Geese (virology), Influenza A Virus, H5N1 Subtype (genetics), Influenza A Virus, H5N1 Subtype (isolation & purification), Mice (MeSH), Neuraminidase (genetics), Orthomyxoviridae Infections (virology), Phylogeny (MeSH), Reassortant Viruses (genetics), Reassortant Viruses (physiology), Sequence Deletion (MeSH), Swine (MeSH), Viral Load (MeSH), Viral Tropism (MeSH).
MESH :
- chemical , genetics : Neuraminidase.
- genetics : Influenza A Virus, H5N1 Subtype, Reassortant Viruses.
- isolation & purification : Influenza A Virus, H5N1 Subtype.
- physiology : Reassortant Viruses.
- virology : Chickens, Ducks, Geese, Orthomyxoviridae Infections.
- Amino Acid Sequence, Animals, Animals, Wild, British Columbia, Evolution, Molecular, Mice, Phylogeny, Sequence Deletion, Swine, Viral Load, Viral Tropism.

Abstract

In the current study, we describe the pathobiologic characteristics of a novel reassortant virus - A/chicken/BC/FAV-002/2015 (H5N1) belonging to clade 2.3.4.4 that was isolated from backyard chickens in British Columbia, Canada. Sequence analyses demonstrate PB1, PA, NA and NS gene segments were of North American lineage while PB2, HA, NP and M were derived from a Eurasian lineage H5N8 virus. This novel virus had a 19 amino acid deletion in the neuraminidase stalk. We evaluated the pathogenic potential of this isolate in various animal models. The virus was highly pathogenic to mice with a LD50 of 10 plaque forming units (PFU), but had limited tissue tropism. It caused only subclinical infection in pigs which did result in seroconversion. This virus was highly pathogenic to chickens, turkeys, juvenile Muscovy ducks (Cairnia moschata foma domestica) and adult Chinese geese (Anser cynoides domesticus) causing a systemic infection in all species. The virus was also efficiently transmitted and resulted in mortality in naïve contact ducks, geese and chickens. Our findings indicate that this novel H5N1 virus has a wide host range and enhanced surveillance of migratory waterfowl may be necessary in order to determine its potential to establish itself in the wild bird reservoir.

DOI: 10.1038/srep23380
PubMed: 26988892
PubMed Central: PMC4796896

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000161
to stream Main, to step Curation: 000161

Le document en format XML

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<author><name sortKey="Joseph, Tomy" sort="Joseph, Tomy" uniqKey="Joseph T" first="Tomy" last="Joseph">Tomy Joseph</name>
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<wicri:regionArea>Animal Health Centre, Ministry of Agriculture, Abbotsford, British Columbia</wicri:regionArea>
<wicri:noRegion>British Columbia</wicri:noRegion>
</affiliation>
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<author><name sortKey="Bowes, Victoria" sort="Bowes, Victoria" uniqKey="Bowes V" first="Victoria" last="Bowes">Victoria Bowes</name>
<affiliation wicri:level="1"><nlm:affiliation>Animal Health Centre, Ministry of Agriculture, Abbotsford, British Columbia, Canada V3G 2M3.</nlm:affiliation>
<country>Canada</country>
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<author><name sortKey="Suderman, Mathew" sort="Suderman, Mathew" uniqKey="Suderman M" first="Mathew" last="Suderman">Mathew Suderman</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</nlm:affiliation>
<country>Canada</country>
<wicri:regionArea>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba</wicri:regionArea>
<wicri:noRegion>Manitoba</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Leung, Anders" sort="Leung, Anders" uniqKey="Leung A" first="Anders" last="Leung">Anders Leung</name>
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<country xml:lang="fr">Canada</country>
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<wicri:noRegion>Manitoba</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cottam Birt, Colleen" sort="Cottam Birt, Colleen" uniqKey="Cottam Birt C" first="Colleen" last="Cottam-Birt">Colleen Cottam-Birt</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</nlm:affiliation>
<country>Canada</country>
<wicri:regionArea>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba</wicri:regionArea>
<wicri:noRegion>Manitoba</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hisanaga, Tamiko" sort="Hisanaga, Tamiko" uniqKey="Hisanaga T" first="Tamiko" last="Hisanaga">Tamiko Hisanaga</name>
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<country>Canada</country>
<wicri:regionArea>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba</wicri:regionArea>
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</author>
<author><name sortKey="Pasick, John" sort="Pasick, John" uniqKey="Pasick J" first="John" last="Pasick">John Pasick</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Animals (MeSH)</term>
<term>Animals, Wild (MeSH)</term>
<term>British Columbia (MeSH)</term>
<term>Chickens (virology)</term>
<term>Ducks (virology)</term>
<term>Evolution, Molecular (MeSH)</term>
<term>Geese (virology)</term>
<term>Influenza A Virus, H5N1 Subtype (genetics)</term>
<term>Influenza A Virus, H5N1 Subtype (isolation & purification)</term>
<term>Mice (MeSH)</term>
<term>Neuraminidase (genetics)</term>
<term>Orthomyxoviridae Infections (virology)</term>
<term>Phylogeny (MeSH)</term>
<term>Reassortant Viruses (genetics)</term>
<term>Reassortant Viruses (physiology)</term>
<term>Sequence Deletion (MeSH)</term>
<term>Swine (MeSH)</term>
<term>Viral Load (MeSH)</term>
<term>Viral Tropism (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Animaux sauvages (MeSH)</term>
<term>Canards (virologie)</term>
<term>Charge virale (MeSH)</term>
<term>Colombie-Britannique (MeSH)</term>
<term>Délétion de séquence (MeSH)</term>
<term>Infections à Orthomyxoviridae (virologie)</term>
<term>Oies (virologie)</term>
<term>Phylogenèse (MeSH)</term>
<term>Poulets (virologie)</term>
<term>Sialidase (génétique)</term>
<term>Souris (MeSH)</term>
<term>Sous-type H5N1 du virus de la grippe A (génétique)</term>
<term>Sous-type H5N1 du virus de la grippe A (isolement et purification)</term>
<term>Suidae (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Tropisme viral (MeSH)</term>
<term>Virus recombinants (génétique)</term>
<term>Virus recombinants (physiologie)</term>
<term>Évolution moléculaire (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Neuraminidase</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Sialidase</term>
<term>Sous-type H5N1 du virus de la grippe A</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Canards</term>
<term>Infections à Orthomyxoviridae</term>
<term>Oies</term>
<term>Poulets</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chickens</term>
<term>Ducks</term>
<term>Geese</term>
<term>Orthomyxoviridae Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Animals, Wild</term>
<term>British Columbia</term>
<term>Evolution, Molecular</term>
<term>Mice</term>
<term>Phylogeny</term>
<term>Sequence Deletion</term>
<term>Swine</term>
<term>Viral Load</term>
<term>Viral Tropism</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Animaux sauvages</term>
<term>Charge virale</term>
<term>Colombie-Britannique</term>
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<term>Phylogenèse</term>
<term>Souris</term>
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<front><div type="abstract" xml:lang="en">In the current study, we describe the pathobiologic characteristics of a novel reassortant virus - A/chicken/BC/FAV-002/2015 (H5N1) belonging to clade 2.3.4.4 that was isolated from backyard chickens in British Columbia, Canada. Sequence analyses demonstrate PB1, PA, NA and NS gene segments were of North American lineage while PB2, HA, NP and M were derived from a Eurasian lineage H5N8 virus. This novel virus had a 19 amino acid deletion in the neuraminidase stalk. We evaluated the pathogenic potential of this isolate in various animal models. The virus was highly pathogenic to mice with a LD50 of 10 plaque forming units (PFU), but had limited tissue tropism. It caused only subclinical infection in pigs which did result in seroconversion. This virus was highly pathogenic to chickens, turkeys, juvenile Muscovy ducks (Cairnia moschata foma domestica) and adult Chinese geese (Anser cynoides domesticus) causing a systemic infection in all species. The virus was also efficiently transmitted and resulted in mortality in naïve contact ducks, geese and chickens. Our findings indicate that this novel H5N1 virus has a wide host range and enhanced surveillance of migratory waterfowl may be necessary in order to determine its potential to establish itself in the wild bird reservoir. </div>
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<DateCompleted><Year>2017</Year>
<Month>01</Month>
<Day>25</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
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<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2045-2322</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>6</Volume>
<PubDate><Year>2016</Year>
<Month>Mar</Month>
<Day>18</Day>
</PubDate>
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<Title>Scientific reports</Title>
<ISOAbbreviation>Sci Rep</ISOAbbreviation>
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<ArticleTitle>Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolated in British Columbia, Canada, 2015.</ArticleTitle>
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<ELocationID EIdType="doi" ValidYN="Y">10.1038/srep23380</ELocationID>
<Abstract><AbstractText>In the current study, we describe the pathobiologic characteristics of a novel reassortant virus - A/chicken/BC/FAV-002/2015 (H5N1) belonging to clade 2.3.4.4 that was isolated from backyard chickens in British Columbia, Canada. Sequence analyses demonstrate PB1, PA, NA and NS gene segments were of North American lineage while PB2, HA, NP and M were derived from a Eurasian lineage H5N8 virus. This novel virus had a 19 amino acid deletion in the neuraminidase stalk. We evaluated the pathogenic potential of this isolate in various animal models. The virus was highly pathogenic to mice with a LD50 of 10 plaque forming units (PFU), but had limited tissue tropism. It caused only subclinical infection in pigs which did result in seroconversion. This virus was highly pathogenic to chickens, turkeys, juvenile Muscovy ducks (Cairnia moschata foma domestica) and adult Chinese geese (Anser cynoides domesticus) causing a systemic infection in all species. The virus was also efficiently transmitted and resulted in mortality in naïve contact ducks, geese and chickens. Our findings indicate that this novel H5N1 virus has a wide host range and enhanced surveillance of migratory waterfowl may be necessary in order to determine its potential to establish itself in the wild bird reservoir. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berhane</LastName>
<ForeName>Yohannes</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Animal Science, University of Manitoba, Winnipeg, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kobasa</LastName>
<ForeName>Darwyn</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Public Health Agency of Canada, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Embury-Hyatt</LastName>
<ForeName>Carissa</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pickering</LastName>
<ForeName>Brad</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Babiuk</LastName>
<ForeName>Shawn</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Immunology, University of Manitoba, Winnipeg, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Joseph</LastName>
<ForeName>Tomy</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Animal Health Centre, Ministry of Agriculture, Abbotsford, British Columbia, Canada V3G 2M3.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bowes</LastName>
<ForeName>Victoria</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>Animal Health Centre, Ministry of Agriculture, Abbotsford, British Columbia, Canada V3G 2M3.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Suderman</LastName>
<ForeName>Mathew</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Leung</LastName>
<ForeName>Anders</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Public Health Agency of Canada, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cottam-Birt</LastName>
<ForeName>Colleen</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hisanaga</LastName>
<ForeName>Tamiko</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pasick</LastName>
<ForeName>John</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada R3E 3M4.</Affiliation>
</AffiliationInfo>
</Author>
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<Language>eng</Language>
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<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>03</Month>
<Day>18</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Sci Rep</MedlineTA>
<NlmUniqueID>101563288</NlmUniqueID>
<ISSNLinking>2045-2322</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>EC 3.2.1.18</RegistryNumber>
<NameOfSubstance UI="D009439">Neuraminidase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000835" MajorTopicYN="N">Animals, Wild</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001955" MajorTopicYN="N">British Columbia</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName>
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<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName>
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<MeshHeading><DescriptorName UI="D005777" MajorTopicYN="N">Geese</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D053124" MajorTopicYN="N">Influenza A Virus, H5N1 Subtype</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
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<MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName>
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Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolated in British Columbia, Canada, 2015.

Pathobiological Characterization of a Novel Reassortant Highly Pathogenic H5N1 Virus Isolated in British Columbia, Canada, 2015.

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